Composite

Part:BBa_K3760011:Design

Designed by: Ha Buer   Group: iGEM21_LZU-CHINA   (2021-10-01)


sgRNA expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a U6 promoter, four DR and three pre-sgRNAs.It must be noted that the mRNA is single-stranded during the sgRNA design.

Source

It does not come from genomic DNA. It is artificially synthesized.

References

[1]Chuang YF, Wang PY, Kumar S, Lama S, Lin FL, Liu GS. Methods for in vitro CRISPR/CasRx-Mediated RNA Editing. Front Cell Dev Biol. 2021;9:667879. Published 2021 Jun 11. doi:10.3389/fcell.2021.667879.
[2]Wessels HH, Méndez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. PMID: 32518401; PMCID: PMC7294996.